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  1. 10 Jan 2025 Journal Article Environments

    Adaptive Degenerate Space-Based Method for Pollutant Source Term Estimation Using a Backward Lagrangian Stochastic Model

    Omri Buchman, Eyal Fattal
    Abstract

    A major challenge in accidental or unregulated releases is the ability to identify the pollutant source, especially if the location is in a large industrial area. Usually in such cases, only a few sensors provide non-zero signal. A crucial issue is therefore the ability to use a small number of sensors in order to identify the source location and rate of emission. The

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  2. 23 Dec 2024 Preprint Preprints

    Purification of an FC-Fusion Protein with [Bathophenathroline: metal] Complexes

    Thisara Jayawickrama Withanage, Ron Alcalay, Olga Krichevsky, Ellen Wachtel, Ohad Mazor, Guy Patchornik
    Abstract

    In this study, we aim at assessing an alternative method for purification of Fc-fusion proteins that does not rely on chromatographic media or ligands. Recombinant human acetylcholinesterase, fused to the Fc-domain of human IgG1 (henceforth, AChE-Fc), was purified with precipitated aromatic complexes composed of the bathophenanthroline (henceforth, batho) chelator with

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  3. 13 Dec 2024 Journal Article Science Advances

    Two-in-one nanoparticle platform induces a strong therapeutic effect of targeted therapies in P-selectin–expressing cancers

    Shani Koshrovski-Michael, Daniel Rodriguez Ajamil, Pradip Dey, Ron Kleiner, Shahar Tevet, Yana Epshtein, Marina Green Buzhor, Rami Khoury, Sabina Pozzi, Gal Shenbach-Koltin, Eilam Yeini, Laura Woythe, ... show all 20 authors
    Abstract

    Combined therapies in cancer treatment aim to enhance antitumor activity. However, delivering multiple small molecules imposes challenges, as different drugs have distinct pharmacokinetic profiles and tumor penetration abilities, affecting their therapeutic efficacy. To circumvent this, poly(lactic-co-glycolic acid) (PLGA)–polyethylene glycol (PEG)–based nanoparticles

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  4. 9 Dec 2024 Journal Article Organics

    Simple and Selective Determination of Free Chlorine in Aqueous Solutions by an Electrophilic Aromatic Substitution Reaction Followed by Liquid Chromatography Coupled with Mass Spectrometry

    Avital Shifrovitch, Moran Madmon, Tamar Shamai Yamin, Avi Weissberg
    Abstract

    We developed a selective technique to rapidly measure free chlorine, which is the sum of elemental chlorine (Cl2), hypochlorous acid (HOCl), and hypochlorite (OCl) in water samples via an electrophilic aromatic substitution reaction hyphenated with liquid chromatography-electrospray ionization tandem mass spectrometry (LC-ESI-MS/MS). Sample preparation involved

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  5. Dec 2024 Journal Article TrAC Trends in Analytical Chemistry

    Metabolite Annotation through Stable Isotope Labeling

    Yonghui Dong, Liron Feldberg, Asaph Aharoni, Uwe Heinig
    Abstract

    Stable isotope labeling (SIL) is a powerful tool for probing cellular metabolism and has been increasingly employed for metabolite annotation in mass spectrometry-based metabolomics. Herein, we outline the principles and methods of designing and performing SIL-based experiments in metabolomics studies. Further, we summarize different SIL approaches and highlight recent

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  6. 30 Oct 2024 Journal Article Rapid Communications in Mass Spectrometry

    Structural elucidation of tramadol, its derivatives, and metabolites using chemical derivatization and liquid chromatography–high-resolution tandem mass spectrometry

    Tamar Shamai Yamin, Avital Shifrovitch, Moran Madmon, Hagit Prihed, Avi Weissberg
    Abstract

    Rationale

    Tramadol (T) is a strong painkiller drug that belongs to the opioid analgesic group. Several accidental intoxication cases after oral administration of T have been reported in the past decade. Tramadol, its derivatives, and metabolites present information-limited mass spectra with one prominent peak representing the amine-containing residue; therefore, their

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  7. 28 Oct 2024 Journal Article Pathogens

    Closing the Gaps: Testing the Efficacy of Carbapenem and Cephalosporin Treatments of Late-Stage Anthrax in Rabbits

    Assa Sittner, Elad Bar-David, Itai Glinert, Amir Ben-Shmuel, Josef Schlomovitz, Haim Levy, Shay Weiss
    Abstract

    Anthrax is a fatal zoonotic disease caused by exposure to Bacillus anthracis spores. The CDC’s guidelines divide anthrax treatment into three categories according to disease progression: post-exposure prophylaxis (PEP), systemic, and systemic with a suspicion of CNS infection. While the prognosis for PEP or the early treatment of systemic anthrax is very good, ingress

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  8. 25 Oct 2024 Preprint Social Science Research Network

    Links between the Indian Ocean Dipole and Persistent Dry Spells in the Eastern Mediterranean Winter

    Sigalit Berkovic, Assaf Hochman
    Abstract

    Persistent Dry Spells (PDS) during winter in the eastern Mediterranean are crucial to understanding the regional challenges of water resources and mitigating agricultural and economic impacts. Winter dry spells significantly affect ecosystem stability, public health, and socioeconomic conditions in a region susceptible to climate variability. Therefore, extending the

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  9. 22 Oct 2024 Journal Article The American Journal of Tropical Medicine and Hygiene

    Case Report: A Novel Virulent Clinical Isolate of Burkholderia pseudomallei Imported from Thailand Exhibiting Resistance to Trimethoprim-Sulfamethoxazole

    Sharon Amit, David Gur, Moshe Aftalion, Asaf Biber, Paula David, Inbar Cohen-Gihon, Hila Cohen, Ron Alcalay, Galia Zaide, Ofir Israeli, Raphael Ber, Shahar Rotem, ... show all 22 authors
    Abstract

    We present the investigation of a Burkholderia pseudomallei strain isolated from the urine of a 56-year-old male returning from a recreational trip to Thailand. The patient initially presented with fever and chills, and lobar as well as bladder and prostate involvement was demonstrated on imaging. Treatment first involved trimethoprim-sulfamethoxazole, the backbone of

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  10. 10 Oct 2024 Journal Article Building and Environment

    Quantifying the impact of vertical greenery systems (VGS) on Mediterranean urban microclimate during heat wave events

    Noa Zuckerman, Nir Shiloah, Itamar M Lensky
    Abstract

    This research investigates the potential of vertical greenery systems (VGS) to modulate the outdoor microclimate in a real-scale Mediterranean climate street canyon with respect to a non-vegetated control during four heat waves and summer conditions. The cooling effect of VGS on air temperature was assessed using an identical set of sensors in both canyons. The effect

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