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  1. 18 Mar 2025 Preprint Preprints

    Purification of Human IgG with Bathophenanthroline Zn2+, Fe2+ or Cu2+ Complexes

    Thisara Jayawickrama Withanage, Ron Alcalay, Olga Krichevsky, Ellen Wachtel, Ohad Mazor, Guy Patchornik
    Abstract

    Pharmaceutical companies are aware of the ongoing effort to satisfy the increasing global demand for therapeutic grade monoclonal antibodies (mAbs), an especially difficult challenge for poor and developing countries. We present a simple, economical, single-step purification approach at neutral pH for polyclonal human IgG (hIgG) that does not require expensive ligands

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  2. 14 Mar 2025 Conference Paper EGU General Assembly 2025

    Links between the Indian Ocean Dipole and Persistent Dry Spells in the Eastern Mediterranean Winter

    Sigalit Berkovic, Assaf Hochman
    Abstract

    Persistent Dry Spells (PDS) during winter in the eastern Mediterranean are crucial to understanding the regional challenges of water resources and mitigating agricultural and economic impacts. Winter dry spells significantly affect ecosystem stability, public health, and socioeconomic conditions in a region susceptible to climate variability. Therefore, extending the

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  3. 12 Mar 2025 Journal Article ACS Applied Materials & Interfaces

    Aminophenol Molecular Capture Layer for Specific Molecular Sensing with Field-Effect Devices

    Pooja Verma, Yuval Ben-Shahar, Soumadri Samanta, Vijay Garika, Shubham Babbar, Shankar Bhattarai, Sherina Harilal, Gil Feldheim, Alexander Pevzner, Ishay Columbus, Hagit Prihed, Evgeny Pikhay, ... show all 20 authors
    Abstract

    There is an urgent need today for interface management with recognition layers composed of short receptor molecules, with excellent specificity and affinity toward a target molecule, for a wide range of sensing applications. The current work demonstrates a specific detection of a G-type nerve agent, which is based on a nucleophilic substitution reaction between the

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  4. 6 Mar 2025 Journal Article Sci

    A Simple Solution for the Inverse Distance Weighting Interpolation (IDW) Clustering Problem

    Nir Benmoshe
    Abstract

    Inverse Distance Weighting (IDW) is a common method for spatial interpolation. Still, its accuracy decreases when there is a cluster of measurement stations or when some measuring stations are hidden behind others. This paper introduces Clusters Unifying Through Hiding Interpolation (CUTHI), a simple approach to enhance IDW accuracy. CUTHI calculates a weight for each

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  5. 26 Feb 2025 Journal Article Bulletin of Atmospheric Science and Technology

    Spatial variability of non-summer daytime boundary layer height over the Israeli coastal-mountain-valley topography reveals unique shallow boundary layer winter events

    Sigalit Berkovic
    Abstract

    The center of Israel presents a relatively simple vertical topographical cross-section: The eastern Mediterranean coast to the west, Judea and Samaria mountains in the center and the deep and steep Jordan valley to the east. Further to the east of the Jordan valley there is a plateau of the Moab mountains.

    This work presents climatological examination

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  6. 4 Feb 2025 Journal Article Antibodies

    Efficient Identification of Monoclonal Antibodies Against Rift Valley Fever Virus Using High-Throughput Single Lymphocyte Transcriptomics of Immunized Mice

    Ronit Rosenfeld, Ron Alcalay, Yfat Yahalom-Ronen, Sharon Melamed, Avital Sarusi-Portuguez, Tal Noy-Porat, Ofir Israeli, Adi Beth-Din, Ronnie Blecher-Gonen, Theodor Chitlaru, Erez Bar-Haim, Tomer Israely, ... show all 14 authors
    Abstract

    Background: Rift Valley fever virus (RVFV) is a zoonotic virus that poses a significant threat to both livestock and human health and has caused outbreaks in endemic regions. In humans, most patients experience a febrile illness; however, in some patients, RVF disease may result in hemorrhagic fever, retinitis, or encephalitis. While several veterinary vaccines are

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  7. 31 Jan 2025 Journal Article Antibodies

    Purification of a Fc-Fusion Protein with [Bathophenathroline: metal] Complexes

    Thisara Jayawickrama Withanage, Ron Alcalay, Olga Krichevsky, Ellen Wachtel, Ohad Mazor, Guy Patchornik
    Abstract

    In this study, we assess an alternative Fc-fusion protein purification method that does not rely on chromatographic media or ligands. Recombinant human acetylcholinesterase, fused to the Fc domain of human IgG1 (henceforth, AChE-Fc), was purified with precipitated aromatic complexes composed of the bathophenanthroline (henceforth, batho) chelator with either Zn2+ or

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  8. 10 Jan 2025 Journal Article Environments

    Adaptive Degenerate Space-Based Method for Pollutant Source Term Estimation Using a Backward Lagrangian Stochastic Model

    Omri Buchman, Eyal Fattal
    Abstract

    A major challenge in accidental or unregulated releases is the ability to identify the pollutant source, especially if the location is in a large industrial area. Usually in such cases, only a few sensors provide non-zero signal. A crucial issue is therefore the ability to use a small number of sensors in order to identify the source location and rate of emission. The

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  9. 13 Dec 2024 Journal Article Science Advances

    Two-in-one nanoparticle platform induces a strong therapeutic effect of targeted therapies in P-selectin–expressing cancers

    Shani Koshrovski-Michael, Daniel Rodriguez Ajamil, Pradip Dey, Ron Kleiner, Shahar Tevet, Yana Epshtein, Marina Green Buzhor, Rami Khoury, Sabina Pozzi, Gal Shenbach-Koltin, Eilam Yeini, Laura Woythe, ... show all 20 authors
    Abstract

    Combined therapies in cancer treatment aim to enhance antitumor activity. However, delivering multiple small molecules imposes challenges, as different drugs have distinct pharmacokinetic profiles and tumor penetration abilities, affecting their therapeutic efficacy. To circumvent this, poly(lactic-co-glycolic acid) (PLGA)–polyethylene glycol (PEG)–based nanoparticles

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  10. 9 Dec 2024 Journal Article Organics

    Simple and Selective Determination of Free Chlorine in Aqueous Solutions by an Electrophilic Aromatic Substitution Reaction Followed by Liquid Chromatography Coupled with Mass Spectrometry

    Avital Shifrovitch, Moran Madmon, Tamar Shamai Yamin, Avi Weissberg
    Abstract

    We developed a selective technique to rapidly measure free chlorine, which is the sum of elemental chlorine (Cl2), hypochlorous acid (HOCl), and hypochlorite (OCl) in water samples via an electrophilic aromatic substitution reaction hyphenated with liquid chromatography-electrospray ionization tandem mass spectrometry (LC-ESI-MS/MS). Sample preparation involved

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