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18 Mar 2025 • Preprint • Preprints
Purification of Human IgG with Bathophenanthroline Zn2+, Fe2+ or Cu2+ Complexes
AbstractPharmaceutical companies are aware of the ongoing effort to satisfy the increasing global demand for therapeutic grade monoclonal antibodies (mAbs), an especially difficult challenge for poor and developing countries. We present a simple, economical, single-step purification approach at neutral pH for polyclonal human IgG (hIgG) that does not require expensive ligands
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14 Mar 2025 • Conference Paper • EGU General Assembly 2025
Links between the Indian Ocean Dipole and Persistent Dry Spells in the Eastern Mediterranean Winter
AbstractPersistent Dry Spells (PDS) during winter in the eastern Mediterranean are crucial to understanding the regional challenges of water resources and mitigating agricultural and economic impacts. Winter dry spells significantly affect ecosystem stability, public health, and socioeconomic conditions in a region susceptible to climate variability. Therefore, extending the
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12 Mar 2025 • Journal Article • ACS Applied Materials & Interfaces
Aminophenol Molecular Capture Layer for Specific Molecular Sensing with Field-Effect Devices
AbstractThere is an urgent need today for interface management with recognition layers composed of short receptor molecules, with excellent specificity and affinity toward a target molecule, for a wide range of sensing applications. The current work demonstrates a specific detection of a G-type nerve agent, which is based on a nucleophilic substitution reaction between the
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6 Mar 2025 • Journal Article • Sci
A Simple Solution for the Inverse Distance Weighting Interpolation (IDW) Clustering Problem
AbstractInverse Distance Weighting (IDW) is a common method for spatial interpolation. Still, its accuracy decreases when there is a cluster of measurement stations or when some measuring stations are hidden behind others. This paper introduces Clusters Unifying Through Hiding Interpolation (CUTHI), a simple approach to enhance IDW accuracy. CUTHI calculates a weight for each
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26 Feb 2025 • Journal Article • Bulletin of Atmospheric Science and Technology
Spatial variability of non-summer daytime boundary layer height over the Israeli coastal-mountain-valley topography reveals unique shallow boundary layer winter events
AbstractThe center of Israel presents a relatively simple vertical topographical cross-section: The eastern Mediterranean coast to the west, Judea and Samaria mountains in the center and the deep and steep Jordan valley to the east. Further to the east of the Jordan valley there is a plateau of the Moab mountains.
This work presents climatological examination
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4 Feb 2025 • Journal Article • Antibodies
Efficient Identification of Monoclonal Antibodies Against Rift Valley Fever Virus Using High-Throughput Single Lymphocyte Transcriptomics of Immunized Mice
AbstractBackground: Rift Valley fever virus (RVFV) is a zoonotic virus that poses a significant threat to both livestock and human health and has caused outbreaks in endemic regions. In humans, most patients experience a febrile illness; however, in some patients, RVF disease may result in hemorrhagic fever, retinitis, or encephalitis. While several veterinary vaccines are
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31 Jan 2025 • Journal Article • Antibodies
Purification of a Fc-Fusion Protein with [Bathophenathroline: metal] Complexes
AbstractIn this study, we assess an alternative Fc-fusion protein purification method that does not rely on chromatographic media or ligands. Recombinant human acetylcholinesterase, fused to the Fc domain of human IgG1 (henceforth, AChE-Fc), was purified with precipitated aromatic complexes composed of the bathophenanthroline (henceforth, batho) chelator with either Zn2+ or
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10 Jan 2025 • Journal Article • Environments
Adaptive Degenerate Space-Based Method for Pollutant Source Term Estimation Using a Backward Lagrangian Stochastic Model
AbstractA major challenge in accidental or unregulated releases is the ability to identify the pollutant source, especially if the location is in a large industrial area. Usually in such cases, only a few sensors provide non-zero signal. A crucial issue is therefore the ability to use a small number of sensors in order to identify the source location and rate of emission. The
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13 Dec 2024 • Journal Article • Science Advances
Two-in-one nanoparticle platform induces a strong therapeutic effect of targeted therapies in P-selectin–expressing cancers
AbstractCombined therapies in cancer treatment aim to enhance antitumor activity. However, delivering multiple small molecules imposes challenges, as different drugs have distinct pharmacokinetic profiles and tumor penetration abilities, affecting their therapeutic efficacy. To circumvent this, poly(lactic-co-glycolic acid) (PLGA)–polyethylene glycol (PEG)–based nanoparticles
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9 Dec 2024 • Journal Article • Organics
Simple and Selective Determination of Free Chlorine in Aqueous Solutions by an Electrophilic Aromatic Substitution Reaction Followed by Liquid Chromatography Coupled with Mass Spectrometry
AbstractWe developed a selective technique to rapidly measure free chlorine, which is the sum of elemental chlorine (Cl2), hypochlorous acid (HOCl), and hypochlorite (OCl−) in water samples via an electrophilic aromatic substitution reaction hyphenated with liquid chromatography-electrospray ionization tandem mass spectrometry (LC-ESI-MS/MS). Sample preparation involved
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