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  1. 18 Dec 2023 Journal Article Frontiers in Marine Science

    Development of long-term primary cell culture of Macrobrachium rosenbergii: morphology, metabolic activity, and cell-cycle analysis

    Gurucharan Sudarshan, Simy Weil, Oron Goldstein, Eliya Sultan, Eliahu D Aflalo, Rivka Ofir, Sean V Zimin, Benyamin Rosental, Amir Sagi
    Abstract

    This study details our efforts to establish a sustainable cell culture of Macrobrachium rosenbergii, involving a 90-day longitudinal study on embryonic primary cell culture. Daily monitoring revealed changes from singular diverse-sized cells attaching on days 4-10 to interconnected cell clusters, transitioning from 2D to combined 2D and 3D structures by days 7-10, leading

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  2. Dec 2023 Journal Article Biological Research

    Functional defects in hiPSCs-derived cardiomyocytes from patients with a PLEKHM2-mutation associated with dilated cardiomyopathy and left ventricular non-compaction

    Nataly Korover, Sharon Etzion, Alexander Cherniak, Tatiana Rabinski, Aviva Levitas, Yoram Etzion, Rivka Ofir, Ruti Parvari, Smadar Cohen
    Abstract

    Dilated cardiomyopathy (DCM) is a primary myocardial disease, leading to heart failure and excessive risk of sudden cardiac death with rather poorly understood pathophysiology. In 2015, Parvari's group identified a recessive mutation in the autophagy regulator, PLEKHM2 gene, in a family with severe recessive DCM and left ventricular non-compaction (LVNC). Fibroblasts

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  3. 12 Nov 2023 Journal Article Sustainable Environment

    Analysis of herbal medicine among Bedouin of the Saint Catherine Protectorate (southern Sinai Peninsula) and its comparison to modern drug design

    Rivka Ofir, Roni Lev, Miriam Ron, Ilan Stavi
    Abstract

    The Saint Catherine region of southern Sinai Peninsula’s drylands encompasses a unique combination of geophysical conditions. These features have formed highly diverse habitats, which support extremely complex plant communities. Throughout history, the local Bedouin population has used many of these plants for therapeutic purposes. The objectives of this study were to

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  4. 16 Aug 2023 Journal Article International Journal of Molecular Sciences

    Special Issue “Stem Cell Biology & Regenerative Medicine”

    Abstract

    More than 50% of pre-clinical studies fail despite a long and expensive journey of drug discovery using animal models [1]. Currently, there is a human-specific replacement: human patient induced pluripotent stem cells (hiPSCs)/human embryonic stem cells (hESCs) and their counterpart corrected cells (using Crispr methodology), which enable the establishment of

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  5. 17 Mar 2023 Journal Article International Journal of Molecular Sciences

    hiPSC-Derived Cells as Models for Drug Discovery 2.0

    Abstract

    Human-induced pluripotent stem cells (hiPSCs) serve as a sustainable resource for studying the molecular foundation of disease development, including initiation and deterioration. Although the process of reprogramming adult cells is accompanied by the obliteration of part of the epigenetic signature, usually upon differentiation of hiPSCs into specific cells, such as

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  6. 9 Jan 2023 Journal Article Frontiers in Marine Science

    Enhanced proliferation in a prawn embryonic primary cell culture ectopically expressing mutated Ras

    Gurucharan Sudarshan, Simy Weil, Noa Rotem-Dai, Rivka Manor, Yariv Greenshpan, Oron Goldstein, Omri Sharabi, Eliahu D Aflalo, Rivka Ofir, Benyamin Rosental, Roi Gazit, Amir Sagi
    Abstract

    Crustacean cell line immortalization has gained a great deal of attention in recent decades for both scientific and applied reasons. Our goal in this study was to advance the state of art towards establishing an immortalized cell line by improving the proliferation rates of primary cells isolated from embryos of the giant freshwater prawn Macrobrachium rosenbergii by

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  7. 17 Dec 2022 Journal Article International Journal of Molecular Sciences

    PLEKHM2 Loss of Function Impairs the Activity of iPSC-Derived Neurons via Regulation of Autophagic Flux

    Hadas Ben-Zvi, Tatiana Rabinski, Rivka Ofir, Smadar Cohen, Gad D Vatine
    Abstract

    Pleckstrin Homology And RUN Domain Containing M2 (PLEKHM2) [delAG] mutation causes dilated cardiomyopathy with left ventricular non-compaction (DCM-LVNC), resulting in a premature death of PLEKHM2[delAG] individuals due to heart failure. PLEKHM2 is a factor involved in autophagy, a master regulator of cellular homeostasis, decomposing pathogens, proteins and other

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  8. 15 Sep 2022 Journal Article Aquaculture

    On genome editing in embryos and cells of the freshwater prawn Macrobrachium rosenbergii

    Jonathan Molcho, Rivka Manor, Maayan Shamsian, Gurucharan Sudarshan, Rivka Ofir, Danit Parker, Simy Weil, Hanin Wattad, Emily Hayun, Tom Levy, Eliahu D Aflalo, Ayal Hendel, ... show all 13 authors
    Abstract

    The clustered regularly interspaced short palindromic repeats (CRISPR) technology provides the means for accurate genomic editing. It has been applied in many kinds of cells and animals for functional genomic studies and for precise selective breeding. Nonetheless, this method has not yet been applied in one of the most important – and well studied – decapod crustacean

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  9. 22 Oct 2021 Journal Article International Journal of Molecular Sciences

    The Effect of Sera from Children with Obstructive Sleep Apnea Syndrome (OSAS) on Human Cardiomyocytes Differentiated from Human Embryonic Stem Cells

    Hen Haddad, Sharon Etzion, Tatiana Rabinski, Rivka Ofir, Danielle Regev, Yoram Etzion, Jacob Gopas, Aviv Goldbart
    Abstract

    Obstructive sleep apnea syndrome (OSAS) patients suffer from cardiovascular morbidity, which is the leading cause of death in this disease. Based on our previous work with transformed cell lines and primary rat cardiomyocytes, we determined that upon incubation with sera from pediatric OSAS patients, the cell’s morphology changes, NF-κB pathway is activated, and their

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  10. 14 Oct 2021 Journal Article Frontiers in Marine Science

    Lentiviral-Transduced Ectopic Expression of Androgenic Hormone in a Crustacean Hematopoietic Primary Cell Culture

    Noa Rotem-Dai, Simy Weil, Yariv Greenshpan, Shai Abehsera, Rivka Manor, Rivka Ofir, Roi Gazit, Benyamin Rosental, Eliahu D Aflalo, Amir Sagi
    Abstract

    Novel monosex biotechnologies in crustacean aquaculture involve the induction of sex reversal through manipulations of the androgenic gland (AG), and its most prominent factor, the insulin-like androgenic gland (IAG) hormone, during early developmental stages. In the giant freshwater prawn Macrobrachium rosenbergii, all-female populations are desirable for aquaculture

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